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货号： R24404

别名： G3PD; GAPD; HEL-S-162eP

应用： WB,IHC-F,IHC-P,ICC/IF

反应种属： Human,Mouse,Rat,Hamster

目录价格：

RMB(含税报价)

数量： - + （库存件）

查看代理商预计货期：现货说明书PDF

产品详情

Product Name

GAPDH Rabbit mAb
Gene ID

2597
SwissProt ID

P04406
Gene Name

GAPDH
Alternative Names

G3PD; GAPD; HEL-S-162eP
Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
Research Field

Neuroscience
Product Categories

Loading Control antibody
Host

Rabbit
Reactivity

Human,Mouse,Rat,Hamster
Application

WB,IHC-F,IHC-P,ICC/IF
Cited

WB (Chicken)
Dilution Ratio

WB: 1/5000-1/10000 IHC: 1/50-1/200 IF: 1/100-1/200
Molecular Weight

Calculated MW: 36 kDa; Observed MW: 36 kDa
Clonality

Monoclonal Antibody
Clonality No.

R08-4C3
Isotype

IgG
Immunogen

Recombinant protein of human GAPDH
Purification

Affinity Purified
Conjugation

Unconjugated
Modification

Unmodified
Form

Liquid
Buffer System

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

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验证详情

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应用缩写

WB:蛋白质印迹法IHC-P:石蜡切片样本的免疫组织化学法IHC-F:冰冻切片样本的免疫组织化学法ICC/IF:免疫细胞荧光 IP:免疫沉淀法FC:流式细胞术ChIP:染色质免疫沉淀法ELISA:酶联免疫吸附测定 713贵宾会检测线路生物产品售后保障范围仅限官网产品详情页中“应用”一栏写明的实验应用，请参照上方的缩写含义，仔细对照后选择合适的产品。

引用文献更多完整详情

标题： The natural product salicin alleviates osteoarthritis progression by binding to IRE1α and inhibiting endoplasmic reticulum stress through the IRE1α-IκBα-p65 signaling pathway

样本种属： Rat

杂志： EXPERIMENTAL AND MOLECULAR MEDICINE

应用： WB

IF： 12.153

标题： Engineered Exosome-Functionalized Extracellular Matrix-Mimicking Hydrogel for Promoting Bone Repair in Glucocorticoid-Induced Osteonecrosis of the Femoral Head

样本种属： Mouse

杂志： ACS Applied Materials & Interfaces

应用： WB

IF： 9.5

标题： Cuproptosis status affects treatment options about immunotherapy and targeted therapy for patients with kidney renal clear cell carcinoma.

样本种属： Human

杂志： Frontiers in Immunology

应用： WB

IF： 8.786

标题： Stem cells from human exfoliated deciduous teeth as an alternative cell source in bio-root regeneration

样本种属： Human

杂志： Theranostics

应用： WB

IF： 8.063

标题： Hypoxia-Induced HIF-1&alpha|| Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis

样本种属： Rat

杂志： Cells

应用： IHC

IF： 7.666

标题： SETD3 is regulated by a couple of microRNAs and plays opposing roles in proliferation and metastasis of hepatocellular carcinoma

样本种属： Mouse,Human

杂志： CLINICAL SCIENCE

应用： WB

IF： 6.124

标题： Polydopamine-Based Resveratrol-Hyaluronidase Nanomedicine Inhibited Pancreatic Cancer Cell Invasive Phenotype in Hyaluronic Acid Enrichment Tumor Sphere Model

样本种属： Human

杂志： ACS Pharmacology & Translational Science

应用： WB

IF： 6

标题： TRAF-STOP alleviates osteoclastogenesis in periodontitis

样本种属： ——

杂志： Frontiers in Pharmacology

应用： WB

IF： 5.988

标题： Hesperetin Exhibits Anti-Inflammatory Effects on Chondrocytes via the AMPK Pathway to Attenuate Anterior Cruciate Ligament Transection-Induced Osteoarthritis

样本种属： Rat

杂志： Frontiers in Pharmacology

应用： WB

IF： 5.811

标题： Inhibition of interleukin-6 trans-signaling improves survival and prevents cognitive impairment in a mouse model of sepsis

样本种属： Mouse

杂志： INTERNATIONAL IMMUNOPHARMACOLOGY

应用： WB

IF： 5.714

标题： Activation of estrogen receptor α inhibits TLR4 signaling in macrophages and alleviates the instability of atherosclerotic plaques in the postmenopausal stage

样本种属： Mouse,Human

杂志： INTERNATIONAL IMMUNOPHARMACOLOGY

应用： IHC,IF,WB

IF： 5.714

标题： Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells

样本种属： Mouse

杂志： FASEB JOURNAL

应用： WB

IF： 5.595

标题： Presegetane diterpenoids from Euphorbia sieboldiana as a new type of anti-liver fibrosis agents that inhibit TGF-β/Smad signaling pathway

样本种属： Human

杂志： BIOORGANIC CHEMISTRY

应用： WB

IF： 5.275

标题： Wuzi-Yanzong-Wan prevents oligoasthenospermia due to TAp73 suppression by affecting cellular junction remodeling in testicular tissue in mice

样本种属： Mouse

杂志： JOURNAL OF ETHNOPHARMACOLOGY

应用： WB

IF： 5.2

标题： Transcription factor Sp1 is upregulated by PKCι to drive the expression of YAP1 during pancreatic carcinogenesis

样本种属： Human

杂志： CARCINOGENESIS

应用： WB

IF： 4.944

标题： Paeoniflorin-loaded pH-sensitive liposomes alleviate synovial inflammation by altering macrophage polarity via STAT signaling

样本种属： Rat

杂志： INTERNATIONAL IMMUNOPHARMACOLOGY

应用： WB

IF： 4.932

标题： Inhibitory effect of the TSG-6 on the BMP-4/Smad signaling pathway and odonto/osteogenic differentiation of dental pulp stem cells

样本种属： Human

杂志： BIOMEDICINE & PHARMACOTHERAPY

应用： WB

IF： 4.545

标题： Ursolic acid protects chondrocytes, exhibits anti-inflammatory properties via regulation of the NF-κB/NLRP3 inflammasome pathway and ameliorates osteoarthritis

样本种属： Rat

杂志： BIOMEDICINE & PHARMACOTHERAPY

应用： WB

IF： 4.545

标题： Effect of F11R Gene Knockdown on Malignant Biological Behaviors of Pancreatic Cancer Cells

样本种属： Human

杂志： Journal of Oncology

应用： WB

IF： 4.501

标题： Desmethoxycurcumin aids IFNα’s anti-HBV activity by antagonising CRYAB reduction and stabilising IFNAR1 protein

样本种属： Human

杂志： JOURNAL OF DRUG TARGETING

应用： WB

IF： 4.5

标题： Safflower yellow alleviates osteoarthritis and prevents inflammation by inhibiting PGE2 release and regulating NF-κB/SIRT1/AMPK signaling pathways

样本种属： Rat

杂志： PHYTOMEDICINE

应用： ICC

IF： 4.268

标题： Construction and validation of a novel ferroptosis-related prognostic signature for lung adenocarcinoma

样本种属： Human

杂志： Translational Lung Cancer Research

应用： WB

IF： 4

标题： Optimal vacuum erectile device therapy regimen for penile rehabilitation in a bilateral cavernous nerve crush rat model

样本种属： Rat

杂志： Andrology

应用： WB,IHC

IF： 3.842

标题： PDGF-AA promotes gap junction intercellular communication in chondrocytes via the PI3K/Akt pathway

样本种属： Mouse

杂志： CONNECTIVE TISSUE RESEARCH

应用： WB

IF： 3.417

标题： Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics

样本种属： Human

杂志： BMC BIOTECHNOLOGY

应用： WB

IF： 3.33

标题： MiR-148a-3p Regulates Skeletal Muscle Satellite Cell Differentiation and Apoptosis via the PI3K/AKT Signaling Pathway by Targeting Meox2

样本种属： Chick

杂志： Frontiers in Genetics

应用： WB

IF： 3.26

标题： Atg3 Overexpression Enhances Bortezomib-Induced Cell Death in SKM-1 Cell

样本种属： Human

杂志： PLoS One

应用： WB

IF： 3.057

标题： Sacral Nerve Stimulation Alleviates Intestinal Inflammation Through Regulating the Autophagy of Macrophages and Activating the Inflammasome Mediated by a Cholinergic Antiinflammatory Pathway in Colitis Rats

样本种属： Rat

杂志： NEUROMODULATION

应用： WB

IF： 3.025

标题： Carvedilol induces pyroptosis through NLRP3-caspase1-ASC inflammasome by nuclear migration of NF-κB in prostate cancer models

样本种属：

杂志： MOLECULAR BIOLOGY REPORTS

应用：

IF： 2.8

标题： Bioinformatics analysis of KLF2 as a potential prognostic factor in ccRCC and association with epithelial‑mesenchymal transition

样本种属： Human

杂志： Experimental and Therapeutic Medicine

应用： WB

IF： 2.751

标题： High Expression Level of TRIP6 is Correlated with Poor Prognosis in Colorectal Cancer and Promotes Tumor Cell Proliferation and Migration

样本种属： Human

杂志： BIOCHEMICAL GENETICS

应用： WB

IF： 2.4

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