Tyramide Signal Amplification (TSA™) from PerkinElmer is a technology that improves sensitivity by up to 100-fold while allowing reduced consumption of primary antibodies or probes. TSA Biotin Kits use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy. The use of TSA reagents results in a significant increase in sensitivity over standard detection methods, while maintaining specificity and resolution. Moreover, TSA reagents allow drastically reduced consumption of primary antibody or probe.
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Reagent1:Goat-HRP anti-mouse/rabbit secondary antibody polymers Reagent2:Streptavidin-HRP Reagent3:TSA-Biotin Reagent4:3% Hydrogen peroxide